Part:BBa_J70032:Design
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 111
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 43
Illegal BsaI.rc site found at 54
Illegal BsaI.rc site found at 69
Design Notes
Can be used to create protein fusions, see BBF RFC15. (The part that links the two BioScaffold parts can be altered as desired, here a 22 bp placeholder is used that leaves at least 7 bp beyond the internal BioBrick scars.) TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when two BioBrick parts are fused together. ^s represent restriction enzyme cut sites.
General Function (after cutting with PpiI and PsrI)
XXXXX XXXXX^XXX TACTAGAG BioScaffold Part BBa_J70032 TACTAGAT XX XXXXX^XXXXX XXXXX^XXXXX XXX ATGATCAC ATGATCTA XX^XXXXX XXXXX
Function when the left and right parts are protein parts (after cutting with PpiI and PsrI)
XXXXX XXXXX^TAA TACTAGAG BioScaffold Part BBa_J70032 TACTAGAT GX XXXXX^XXXXX XXXXX^XXXXX ATT ATGATCAC ATGATCTA CX^XXXXX XXXXX
Function when the left and right parts are protein parts (after cutting with PpiI and PsrI,) with appropriately positioned BamHI sites.
XXXXG GATCC^TAA TACTAGAG BioScaffold Part BBa_J70032 TACTAGAT GG GATCC^XXXXX XXXXC^CTAGG ATT ATGATCAC ATGATCTA CC^CTAGG XXXXX
What happens after cutting with MabI and purifying with either gel purification or a Qiagen PCR cleanup spin column
General Case
XXXXX XXXXX^ ^XXXXX XXXXX^ ^XXXXX XXXXX
Case when left and right parts are a protein part
XXXXX XXXXX^ ^XXXXX XXXXX^ ^XXXXX XXXXX
Case when left and right parts are protein parts with appropriately positioned BamHI sites
XXXXG GATCC^ ^XXXXX XXXXC^ ^CTAGG XXXXX
Source
Designed